The Bradford Assay is a colorimetric protein assay used to determine protein concentration, based on an absorbance shift of the dye Coomassie Brilliant Blue G-250 when it binds to proteins. It is a quick, cost-effective method, sensitive to microgram quantities, yet can be influenced by the presence of detergents and other chemicals in samples.

Bradford Assay

colorimetric protein assay

Protein concentration is a crucial measurement in biochemistry and molecular biology, indicating the amount of protein present in a solution. Accurate determination of protein concentration is essential for downstream experiments and applications involving quantitative proteomics, enzyme kinetics, and therapeutic drug design.

protein concentration

absorbance shift

Coomassie Brilliant Blue G-250 is a dye commonly used for the colorimetric detection and quantification of proteins in techniques such as the Bradford assay. It binds to proteins, particularly arginine residues, and undergoes a shift in absorption maximum, leading to a blue coloration that can be easily quantified using spectrophotometric methods.

Coomassie Brilliant Blue G-250

Binding to proteins

Quick method

cost-effective method

sensitive to microgram quantities

Influence of detergents

Influence of chemicals in samples

Types of Protein Assays

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