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Concept
Absorbance Shift
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Bradford Assay
The
Bradford Assay
is a
colorimetric protein assay
used to determine
protein concentration
, based on an
absorbance shift
of the dye
Coomassie Brilliant Blue G-250
when it binds to proteins. It is a quick,
cost-effective method
, sensitive to microgram quantities, yet can be influenced by the presence of detergents and other chemicals in samples.
3