The T7 promoter system is a powerful tool used in molecular biology for expressing high levels of recombinant proteins in bacterial cells, particularly E. coli. It relies on the T7 bacteriophage's RNA polymerase to drive gene expression, allowing precise control and high efficiency in producing target proteins.

T7 Promoter System

Molecular biology is a branch of science that explores the structure and function of the molecules essential to life, focusing on the interactions between various systems of a cell, including the interrelationship of DNA, RNA, and protein synthesis. It provides insights into the molecular mechanisms of genetic replication, transcription, translation, and cell function, forming the foundation for understanding complex biological processes and disease mechanisms.

Molecular Biology

Recombinant proteins

Bacterial cells are unicellular microorganisms that lack a nucleus and possess a membrane-bound cell wall, playing crucial roles in both ecosystem functioning and human health. With their rapid reproductive abilities and diverse metabolic processes, bacteria are essential for nutrient cycling, fermentation, and biotechnological applications.

Bacterial Cells

E. Coli

T7 bacteriophage

RNA polymerase is an essential enzyme responsible for transcribing DNA into RNA, a critical step in gene expression. It plays a pivotal role in synthesizing messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA), each crucial for protein synthesis and cellular function.

RNA Polymerase

Gene expression is the process by which information from a gene is used to synthesize a functional gene product, typically proteins, which ultimately determine cellular function and phenotype. This process is tightly regulated at multiple levels, including transcription, RNA processing, translation, and post-translational modifications, to ensure proper cellular function and response to environmental cues.

Concepts

Your Lessons