Phosphatidylinositol (3,4,5)-trisphosphate (PIP3) is a critical phospholipid that acts as a second messenger in various cellular signaling pathways, particularly the PI3K/AKT pathway, which is essential for cell growth, survival, and metabolism. PIP3 is generated by the phosphorylation of phosphatidylinositol (4,5)-bisphosphate (PIP2) by phosphoinositide 3-kinases (PI3Ks) and is dephosphorylated by the tumor suppressor PTEN, highlighting its role in cancer and other diseases when dysregulated.