Nitrocellulose membrane is a highly versatile material widely used in molecular biology for the immobilization of proteins, nucleic acids, and other biomolecules due to its high binding capacity and compatibility with various detection methods. Its porous structure allows for efficient transfer and binding of molecules, making it indispensable in techniques such as Western blotting, Southern blotting, and dot blot assays.

Nitrocellulose Membrane

Nitrocellulose membrane

Molecular biology is a branch of science that explores the structure and function of the molecules essential to life, focusing on the interactions between various systems of a cell, including the interrelationship of DNA, RNA, and protein synthesis. It provides insights into the molecular mechanisms of genetic replication, transcription, translation, and cell function, forming the foundation for understanding complex biological processes and disease mechanisms.

molecular biology

immobilization of proteins

Immobilization of nucleic acids

Biomolecules are organic molecules that are essential to the structure and function of living organisms, including proteins, nucleic acids, carbohydrates, and lipids. They play critical roles in biological processes such as catalysis, signaling, and structural support, and are fundamental to understanding cellular and molecular biology.

biomolecules

high binding capacity

Detection methods refer to the techniques and processes used to identify the presence or absence of a specific substance, condition, or phenomenon. These methods are crucial in fields like medicine, security, environmental science, and technology, where accurate and timely detection can have significant implications for decision-making and outcomes.

detection methods

porous structure

efficient transfer and binding of molecules

Western blotting is a widely used analytical technique in molecular biology and biochemistry to detect specific proteins in a sample through gel electrophoresis followed by transfer to a membrane and detection using antibodies. This method allows for the qualitative and semi-quantitative analysis of protein expression, post-translational modifications, and interactions.

Western blotting

Southern blotting is a molecular biology technique used to detect specific DNA sequences within a complex mixture of DNA. It involves the transfer of DNA fragments from an agarose gel to a membrane, followed by hybridization with a labeled probe that binds to the target sequence.

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