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Ligand binding assays
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Summary
Ligand binding assays
are essential
Techniques in biochemistry
and pharmacology used to measure the
Interaction between a ligand and its target molecule
, providing critical insights into
Binding Affinity
, kinetics, and the
Molecular Mechanisms
underlying these interactions. These assays are pivotal for
Drug Discovery
, enabling the identification and
Optimization of compounds
that can modulate
Biological Targets
effectively.
Concepts
Binding Affinity
Kinetics Of Ligand Binding
Receptor-ligand Interaction
Equilibrium dissociation constant (Kd)
Saturation Binding
Competitive Binding Assays
Non-competitive Binding
Scatchard Plot Analysis
Radioligand Binding Assays
Fluorescence Polarization
Surface Plasmon Resonance (SPR)
Isothermal Titration Calorimetry (ITC)
High-Throughput Screening (HTS)
Pharmacodynamics
Molecular Docking
Protein Microarrays
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