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Concept
Fluorescence-based Assays
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Summary
Fluorescence-based Assays
are
Analytical Techniques
that utilize the
Emission of light
by a substance that has
Absorbed Light
or other
Electromagnetic Radiation
to measure the
Presence or concentration of biomolecules
. These assays are highly sensitive, allowing for the
Detection of low-abundance targets
in
Complex biological samples
, and are widely used in
Research and clinical diagnostics
.
Concepts
Fluorescence
Fluorophore
Spectroscopy
Signal-to-Noise Ratio
Quantum Yield
Emission Spectrum
Excitation Wavelength
Photobleaching
Fluorescence Resonance Energy Transfer (FRET)
Time-resolved Fluorescence
Microplate Reader
High-Throughput Screening
Biomolecular Interaction
Cell Viability Assays
Relevant Degrees
General Toxicology 100%
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