A double reciprocal plot, also known as a Lineweaver-Burk plot, is a graphical representation used to analyze enzyme kinetics by linearizing the Michaelis-Menten equation. It provides a straightforward way to determine key kinetic parameters, such as the maximum reaction rate (Vmax) and the Michaelis constant (Km), by plotting the reciprocal of reaction velocity against the reciprocal of substrate concentration.