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Biomolecule Purification
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Summary
Biomolecule Purification
is a
Critical Process
in biochemistry and
Molecular Biology
, aimed at
Isolating a single type of biomolecule
from a
Complex mixture
. This process is essential for studying the structure, function, and
Interactions of biomolecules
, enabling
Advancements in research
, diagnostics, and
Therapeutic Development
.
Concepts
Chromatography
Electrophoresis
Centrifugation
Filtration
Precipitation
Dialysis
Affinity Purification
Size-exclusion Chromatography
Ion-exchange Chromatography
Hydrophobic Interaction Chromatography
Reversed-phase Chromatography
Gel Electrophoresis
SDS-PAGE
2D Electrophoresis
Ultracentrifugation
Differential Centrifugation
Density Gradient Centrifugation
Membrane Filtration
Ultrafiltration
Crystallization
Liquid-Liquid Extraction
Solid-Phase Extraction
Protein Purification
Nucleic Acid Purification
Enzyme Purification
Antibody Purification
Cell Disruption
Aqueous Two-phase Systems
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