RNA purification is a critical process in molecular biology that involves isolating RNA from biological samples to ensure its integrity and purity for downstream applications like sequencing, RT-PCR, and gene expression analysis. Effective RNA purification requires careful handling to prevent degradation by RNases and often involves techniques such as organic extraction, column-based methods, or magnetic bead-based protocols.

RNA Purification

RNA purification

Molecular biology is a branch of science that explores the structure and function of the molecules essential to life, focusing on the interactions between various systems of a cell, including the interrelationship of DNA, RNA, and protein synthesis. It provides insights into the molecular mechanisms of genetic replication, transcription, translation, and cell function, forming the foundation for understanding complex biological processes and disease mechanisms.

molecular biology

isolating RNA

Biological samples are critical for understanding and analyzing the biological processes of organisms, enabling advancements in fields such as medicine, genetics, and environmental science. These samples, which include tissues, cells, blood, and other bodily fluids, must be collected, stored, and processed under specific conditions to maintain their integrity and reliability for research and diagnostic purposes.

biological samples

integrity and purity

downstream applications

sequencing

RT-PCR

Gene expression analysis is a powerful tool used to understand the functional elements of the genome by measuring the activity (expression) levels of thousands of genes simultaneously. It helps in identifying the molecular mechanisms underlying various biological processes and diseases, facilitating the development of targeted therapies and personalized medicine.

gene expression analysis

RNA degradation is a crucial cellular process that regulates gene expression by controlling the levels of RNA molecules, thus influencing protein synthesis and cellular responses. This process involves a variety of mechanisms and pathways that ensure the removal of faulty, unnecessary, or excess RNA, maintaining cellular homeostasis and function.

CUSTOMIZE YOUR LEARNING

CUSTOMIZE YOUR LEARNING