Native PAGE is a type of polyacrylamide gel electrophoresis that allows proteins to be separated based on their size and native charge without denaturing them, preserving their biological activity and interactions. This technique is crucial for studying protein complexes and interactions as it maintains the proteins in their functional state throughout the analysis.

Native PAGE

Polyacrylamide gel electrophoresis (PAGE) is a technique used to separate proteins or nucleic acids based on their size and charge, utilizing a polyacrylamide gel as the separation medium. It is highly effective for analyzing complex mixtures, allowing for the precise identification and comparison of molecular components.

polyacrylamide gel electrophoresis

Proteins are complex macromolecules essential for various biological functions, including catalyzing metabolic reactions, DNA replication, and transporting molecules. They are composed of amino acids linked in a specific sequence, which determines their structure and function.

proteins

Size separation

native charge

Denaturing proteins

Biological activity refers to the effect a substance has on living organisms, tissues, or cells, and is a critical measure in pharmacology and toxicology for assessing the efficacy and safety of drugs and chemicals. It encompasses a wide range of interactions, including enzyme inhibition, receptor binding, and gene expression modulation, which are crucial for understanding the mechanism of action of bioactive compounds.

biological activity

Protein interactions are fundamental to virtually all biological processes, as they dictate the formation of protein complexes that perform cellular functions. Understanding these interactions is crucial for insights into cellular mechanisms and the development of therapeutic interventions for diseases.

Protein Interactions

Protein complexes are assemblies of multiple protein molecules that interact to perform specific biological functions, often exhibiting properties that are not present in individual proteins. These complexes are essential for a wide range of cellular processes, including signal transduction, DNA replication, and metabolic pathways, and their formation and regulation are critical for maintaining cellular homeostasis.

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