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A microplate, also known as a microtiter plate, is a flat plate with multiple wells used as small test tubes in laboratory experiments, particularly in high-throughput screening and diagnostic testing. Its design allows simultaneous handling of multiple samples, enhancing efficiency in biochemical assays and reducing reagent consumption.
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High-Throughput Screening (HTS) is a method used in drug discovery to quickly conduct millions of chemical, genetic, or pharmacological tests through automation and miniaturization, allowing researchers to rapidly identify active compounds, antibodies, or genes that modulate a particular biomolecular pathway. This approach accelerates the process of finding potential therapeutic agents by efficiently evaluating large libraries of compounds against biological targets.
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Biochemical assays are analytical methods used to measure the concentration or activity of a biological molecule, providing crucial insights into biochemical processes and aiding in drug development. They are essential tools in research and diagnostics, offering quantitative and qualitative data on enzyme activity, protein interactions, and cellular functions.
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Diagnostic testing is a critical component in healthcare that involves the use of tests and procedures to identify the presence of a disease or condition. It helps in guiding treatment decisions, monitoring disease progression, and assessing the effectiveness of interventions.
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A microplate reader is a special machine that helps scientists see tiny things by shining light on them and seeing how the light changes. It's like a super detective that can tell us what's in a tiny drop of liquid by looking at how it plays with light.
Enzyme-Linked Immunosorbent Assay (ELISA) is a highly sensitive and specific biochemical technique used to detect the presence of antigens or antibodies in a sample. It utilizes enzyme-linked antibodies and colorimetric detection to quantify the target molecule, making it a staple in diagnostics and research for detecting diseases and monitoring immune responses.
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Spectrophotometry is a quantitative measurement technique used to determine the concentration of a substance by measuring the intensity of light absorbed at specific wavelengths. It is widely used in chemistry, biology, and physics to analyze the spectral properties of substances and to monitor reactions and processes in real-time.
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Fluorescence detection is a sensitive analytical technique that measures the emission of light by a substance that has absorbed light, typically used for identifying and quantifying biological and chemical substances. It is widely employed in various fields such as biochemistry, molecular biology, and medical diagnostics due to its high specificity and sensitivity.
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Cell culture is a laboratory technique where cells are grown under controlled conditions, typically outside of their natural environment, to study their behavior, function, and response to various stimuli. This method is essential for biological research, drug development, and the production of vaccines and therapeutic proteins.
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Sample handling is the process of collecting, transporting, storing, and preparing samples for analysis to ensure their integrity and reliability. Proper Sample handling is crucial to avoid contamination, degradation, or alteration, which could lead to inaccurate results and conclusions.
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Automation in laboratories means using machines and computers to help scientists do their work faster and better. This helps them find answers to their questions more quickly and safely, like a robot helper in science class.
Enzyme-linked immunosorbent assay (ELISA) is a widely used analytical biochemistry technique to detect and quantify proteins, hormones, antibodies, and antigens in biological samples. The method relies on antigen-antibody interactions and an enzyme-mediated color change to provide a measurable signal correlating with the presence and concentration of the target molecule.
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Indirect ELISA is a sensitive and versatile assay used to detect the presence of specific antibodies in a sample by utilizing a secondary antibody conjugated to an enzyme. This method amplifies the signal and provides greater flexibility in terms of antibody selection, making it suitable for a wide range of applications including diagnostics and research.
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Enzyme-linked immunosorbent assay (ELISA) is a powerful laboratory technique used to detect and quantify soluble substances, such as proteins, peptides, antibodies, and hormones. It relies on specific antigen-antibody interactions and uses enzyme-linked detection for signal amplification and visualization, making it a highly sensitive and specific method for various applications in research and diagnostics.
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