Matrix-assisted laser desorption/ionization (MALDI) is a soft ionization technique used in mass spectrometry, allowing for the analysis of large biomolecules by embedding them in a matrix that absorbs laser energy, facilitating their ionization without fragmentation. This method is essential for identifying and characterizing proteins, peptides, and other macromolecules in complex mixtures, making it invaluable in proteomics and biomolecular research.