cDNA synthesis is the process of creating complementary DNA (cDNA) from an RNA template using the enzyme reverse transcriptase. This technique is essential for studying gene expression and is a crucial step in applications such as RT-PCR and RNA sequencing.

CDNA Synthesis

cDNA synthesis

complementary DNA

An RNA template is a strand of RNA that serves as a guide for the synthesis of complementary molecules, such as DNA or another RNA strand, during processes like transcription and reverse transcription. This template mechanism is crucial for the accurate transmission of genetic information and the regulation of gene expression.

RNA template

Reverse transcriptase is an enzyme that catalyzes the transcription of RNA into DNA, a process contrary to the usual flow of genetic information. It is crucial in the life cycle of retroviruses, like HIV, and is a target for antiviral drugs.

reverse transcriptase

Gene expression is the process by which information from a gene is used to synthesize a functional gene product, typically proteins, which ultimately determine cellular function and phenotype. This process is tightly regulated at multiple levels, including transcription, RNA processing, translation, and post-translational modifications, to ensure proper cellular function and response to environmental cues.

gene expression

RT-PCR

RNA sequencing is a powerful technique used to analyze the quantity and sequences of RNA in a sample, providing insights into gene expression and regulation. It has revolutionized genomics by enabling the comprehensive profiling of transcriptomes, aiding in the understanding of complex biological processes and diseases.

CUSTOMIZE YOUR LEARNING

CUSTOMIZE YOUR LEARNING

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